| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Frost, Hope C (12)
|
VADIG |
33.40 |
34.74 |
1.34 |
| 2 |
Ashby, Leah P (12)
|
VADIG |
37.47 |
37.49 |
0.02 |
| 3 |
Lohr, Faith M (12)
|
VA757 |
|
41.34 |
- |
| 4 |
Timberg, Emma C (12)
|
VA757 |
|
43.86 |
- |
| 5 |
Timberg, Mia A (11)
|
VA757 |
|
45.87 |
- |
| 6 |
Strite, Carter E (11)
|
VARACE |
|
46.08 |
- |
| 7 |
Heyward, Alexandra P (11)
|
VAUN75 |
|
46.32 |
- |
| 8 |
Shearer, Jacquelyn M (11)
|
VA757 |
|
46.85 |
- |
| 9 |
Dodson, Sadie (11)
|
VA757 |
|
47.76 |
- |
| 10 |
Martin, Avery L (12)
|
VA757 |
|
48.46 |
- |
| 11 |
Masengale, Charlotte M (12)
|
VA757 |
|
49.83 |
- |
| 12 |
Olsen, Sarah A (11)
|
VA757 |
|
50.57 |
- |
| 13 |
Wester, Madison E (11)
|
VA757 |
|
54.80 |
- |
|
Monestine, Ymanee R (12)
|
VAUN-Q |
|
DQ |
- |