| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Caputo, Quinn M (13)
|
PVNCAP |
59.23 |
56.40 |
-2.83 |
| 2 |
Miceli, Jack M (13)
|
PVNCAP |
58.11 |
56.53 |
-1.58 |
| 3 |
Harrington, Charlie W (13)
|
VANOVA |
57.92 |
56.88 |
-1.04 |
| 4 |
Kominski, Tyler M (13)
|
PVNCAP |
1:00.77 |
58.81 |
-1.96 |
| 5 |
Simons, Matthew A (13)
|
MRLIAC |
1:01.28 |
59.73 |
-1.55 |
| 6 |
Redfern, Cooper C (13)
|
VANOVA |
1:01.84 |
59.87 |
-1.97 |
| 7 |
Jin, Hanchi (13)
|
MRLIAC |
1:01.28 |
1:00.49 |
-0.79 |
| 8 |
Harrington, Cullen S (13)
|
VANOVA |
1:03.45 |
1:00.84 |
-2.61 |
| 9 |
Valentine, Jack M (13)
|
VATIDE |
1:02.97 |
1:01.08 |
-1.89 |
| 10 |
Zhu, Jia Le (13)
|
MRLIAC |
1:09.29 |
1:02.15 |
-7.14 |
| 11 |
Dionne, Luc S (13)
|
PVNCAP |
1:02.48 |
1:02.25 |
-0.23 |
| 12 |
Macko, John (13)
|
MRLIAC |
1:05.31 |
1:02.48 |
-2.83 |
| 13 |
Panos, Alexander N (13)
|
VANOVA |
1:02.93 |
1:02.69 |
-0.24 |
| 14 |
Council, Jaedan Z (13)
|
VATIDE |
1:07.40 |
1:05.42 |
-1.98 |
| 15 |
Chamberlain, Kaden E (13)
|
VATIDE |
1:08.41 |
1:05.88 |
-2.53 |
| 16 |
Morrozoff, Ari A (13)
|
VATIDE |
1:07.60 |
1:07.42 |
-0.18 |