| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fuller, Abigail B (13)
|
VAACAC |
28.79 |
29.41 |
0.62 |
| 2 |
Simmons, Samantha E (13)
|
VANOVA |
30.53 |
30.29 |
-0.24 |
| 3 |
Semo, Kylie E (13)
|
VANOVA |
30.75 |
30.50 |
-0.25 |
| 4 |
Masterson, Allison M (14)
|
VATSU |
33.49 |
30.57 |
-2.92 |
| 5 |
DeGuzman, Zoe B (14)
|
VAACAC |
31.48 |
30.60 |
-0.88 |
| 6 |
Gohn, Emily C (14)
|
VABASS |
31.58 |
31.02 |
-0.56 |
| 7 |
Kluger, Pearl L (13)
|
VAWAC |
30.83 |
31.03 |
0.20 |
| 8 |
Kiger, Ally S (14)
|
VAWAC |
33.20 |
31.24 |
-1.96 |
| 9 |
Hanson, Isabelle A (14)
|
VAWAC |
30.77 |
31.46 |
0.69 |
| 10 |
Pataky, Ellen C (14)
|
VATSU |
|
32.05 |
- |
| 11 |
Gann, Adrianna E (14)
|
VAWAC |
31.64 |
32.16 |
0.52 |
| 12 |
Silva, Veronica Y (13)
|
VANOVA |
32.50 |
32.18 |
-0.32 |
| 13 |
Masterson, Caroline A (13)
|
VATSU |
31.12 |
32.54 |
1.42 |
| 14 |
Calder, Paxton G (13)
|
VANOVA |
34.06 |
33.89 |
-0.17 |
| 15 |
Crampton, Isabella E (13)
|
VATSU |
34.20 |
34.80 |
0.60 |
| 16 |
Young, Kelly E (14)
|
VAACAC |
35.33 |
34.93 |
-0.40 |
| 17 |
McMahan, Isabelle E (13)
|
VAWAC |
34.62 |
35.83 |
1.21 |
|
Wright, Alex M (13)
|
VANOVA |
31.28 |
NS |
- |
|
Rakes, Anna M (13)
|
VANOVA |
32.49 |
NS |
- |