| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DiGiacomo, Sirena G (12)
|
VASHRK |
32.64 |
32.03 |
-0.61 |
| 2 |
Caten, Margaret M (12)
|
VASEAL |
30.36 |
32.52 |
2.16 |
| 3 |
Shindler, Julianne E (11)
|
VASEAL |
|
37.55 |
- |
| 4 |
Spalding, Elizabeth E (11)
|
VACUDA |
|
40.02 |
- |
| 5 |
Fulco, Sophia J (11)
|
VACUDA |
|
40.55 |
- |
| 6 |
Walker, Madison N (11)
|
VASHRK |
43.60 |
42.07 |
-1.53 |
| 7 |
Foose, Ingrid H (12)
|
VASHRK |
|
42.77 |
- |
| 8 |
Cuce, Elianna G (11)
|
VASHRK |
|
43.83 |
- |
| 9 |
Seaborn, Mary A (12)
|
VADLPH |
44.31 |
46.10 |
1.79 |
| 10 |
Mackey, Micah F (11)
|
VASEAL |
46.10 |
47.64 |
1.54 |
|
Haislip, Madison L (11)
|
VADLPH |
|
DQ |
- |
|
Djordjevic, Tijana (11)
|
VASEAL |
54.64 |
NS |
- |