| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sykes, Katharine E (10)
|
VAWAC |
37.75 |
39.10 |
1.35 |
| 2 |
Hynst, Lexi M (10)
|
VASRVA |
|
40.28 |
- |
| 3 |
Parks, Victoria Z (10)
|
VAWAC |
42.04 |
41.31 |
-0.73 |
| 4 |
Pozzi, Anais L (10)
|
VASRVA |
43.05 |
42.44 |
-0.61 |
| 5 |
Statts, Charlotte V (10)
|
VASRVA |
42.54 |
43.07 |
0.53 |
| 6 |
Eaker, Carter G (9)
|
VAYOBX |
45.15 |
45.03 |
-0.12 |
| 7 |
Bradbury, Catherine W (9)
|
VASRVA |
|
45.60 |
- |
| 8 |
Wagner, Madelyn G (9)
|
VASRVA |
50.40 |
48.18 |
-2.22 |
| 9 |
Seibert, Skylar M (9)
|
VAYOBX |
49.75 |
49.11 |
-0.64 |
| 10 |
Sullivan, Mckenzie A (10)
|
VAWAC |
52.53 |
51.14 |
-1.39 |
| 11 |
Oltra Render, Elena M (9)
|
VAWAC |
55.21 |
53.25 |
-1.96 |
| 12 |
Meredith, Charlotte (6)
|
VASRVA |
1:06.79 |
1:00.99 |
-5.80 |
| 13 |
Zhang, Madeline (8)
|
VASRVA |
|
1:02.73 |
- |
| 14 |
DesMarteau, Tara M (6)
|
VASRVA |
1:04.74 |
1:04.06 |
-0.68 |
|
Flores, Teresa (8)
|
VASRVA |
|
NS |
- |