| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vanyo, Vivian R (6)
|
VAUNAT |
|
50.23 |
- |
| 2 |
Wells, Emily M (8)
|
VACYAC |
50.08 |
51.88 |
1.80 |
| 3 |
Dunn, Virginia M (8)
|
VADC |
|
52.46 |
- |
| 3 |
Wong, Kaitlyn (7)
|
VAWAC |
|
52.46 |
- |
| 5 |
Bailey, Mackenzie S (7)
|
VANOVA |
1:06.26 |
52.82 |
-13.44 |
| 6 |
Robertson, Caroline C (8)
|
VAWAC |
|
55.87 |
- |
| 7 |
Keener, Caroline M (8)
|
VANOVA |
1:07.44 |
58.88 |
-8.56 |
| 8 |
Mullinax, Rosina G (8)
|
VADC |
1:14.86 |
1:00.20 |
-14.66 |
| 9 |
Royster, Brielle V (6)
|
VANOVA |
1:10.91 |
1:01.18 |
-9.73 |
| 10 |
Currier, Hailey M (7)
|
VAWAC |
1:07.91 |
1:02.18 |
-5.73 |
| 11 |
Hitt, Virginia-Annabelle H (7)
|
VACYAC |
1:09.71 |
1:03.04 |
-6.67 |
| 12 |
Ferguson, Harper R (8)
|
VASRVA |
|
1:05.49 |
- |
| 13 |
McDorman, Erica G (8)
|
VANOVA |
1:08.94 |
1:05.86 |
-3.08 |
| 14 |
Williamson, Annika R (8)
|
VASRVA |
1:10.89 |
1:10.11 |
-0.78 |
| 15 |
George, Audrey L (7)
|
VANOVA |
|
1:20.53 |
- |