| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Chloe A (9)
|
NIGCOM |
52.46 |
50.89 |
-1.57 |
| 2 |
Martin, Meghan H (9)
|
NIWEBS |
57.00 |
52.28 |
-4.72 |
| 3 |
Ritter, Madeleine (7)
|
NIPACK |
|
55.88 |
- |
| 4 |
Huerta-Andrade, Amanda (9)
|
NIFAST |
1:05.93 |
1:00.30 |
-5.63 |
| 5 |
Anthony, Jillian M (9)
|
NIPMSC |
1:03.12 |
1:00.49 |
-2.63 |
| 6 |
van Wichen, Jessica D (9)
|
NIWEBS |
1:03.82 |
1:03.69 |
-0.13 |
| 7 |
Phillips, Hayley Q (9)
|
NIIRON |
1:15.12 |
1:06.84 |
-8.28 |
| 8 |
Buzzell, Emily H (8)
|
NIGRSC |
|
1:09.98 |
- |
| 9 |
Crowley, Lila M (8)
|
NIGRSC |
|
1:11.33 |
- |
| 10 |
Mantell, Norah A (7)
|
NIIRON |
|
1:11.47 |
- |
| 11 |
Garigen, Natalie K (7)
|
NIWEBS |
|
1:14.03 |
- |
| 12 |
Thayse, Eden A (9)
|
NISDSC |
1:20.43 |
1:14.63 |
-5.80 |
| 13 |
Considine, Elizabeth A (8)
|
NIWEBS |
|
1:29.00 |
- |
| 14 |
Ashby, Emma M (8)
|
NISDSC |
|
1:32.05 |
- |
|
Farrell, Kamryn L (6)
|
NIGRSC |
|
DQ |
- |
|
Manciocchi, Kayden M (8)
|
NIGRSC |
|
DQ |
- |
|
Gonzalez, Alexis W (8)
|
NIPACK |
|
DQ |
- |
|
LaDelfa, Sophia L (9)
|
NIWEBS |
|
DQ |
- |