| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trapp, Angelina L (12)
|
NIPMSC |
29.73 |
31.92 |
2.19 |
| 2 |
Newsome, Amber G (12)
|
NIPMSC |
33.06 |
32.46 |
-0.60 |
| 3 |
Share, Elizabeth A (11)
|
NIPMSC |
32.06 |
32.49 |
0.43 |
| 4 |
Geary, Kaitlyn J (12)
|
NIMERC |
31.95 |
32.80 |
0.85 |
| 5 |
Nguessamble, Tristyn D (12)
|
NISDSC |
34.85 |
34.32 |
-0.53 |
| 6 |
Lamb, Emily A (12)
|
NIMERC |
41.42 |
35.60 |
-5.82 |
| 7 |
Thurston, Lauren A (11)
|
NIGCOM |
41.59 |
39.50 |
-2.09 |
| 8 |
Zeller, Sara L (12)
|
NISDSC |
42.37 |
47.61 |
5.24 |
|
Bartholomay, Meghan E (12)
|
NIGCOM |
32.90 |
NS |
- |
|
Huerta-Andrade, Isabella (12)
|
NIPACK |
32.25 |
NS |
- |