| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Klafehn, Bailey R (18)
|
NIVICT |
2:10.62 |
2:21.80 |
11.18 |
| 2 |
Wood, Alicia G (14)
|
NIIRON |
2:18.85 |
2:25.45 |
6.60 |
| 3 |
Roperti, Pearl M (14)
|
NISEAS |
2:25.10 |
2:30.44 |
5.34 |
| 4 |
Federico, Sydney A (15)
|
NIGRSC |
2:28.64 |
2:31.83 |
3.19 |
| 5 |
Reitz, Meghan E (17)
|
NIHAST |
2:20.15 |
2:36.38 |
16.23 |
| 6 |
Tucker, Abby C (13)
|
NIFAST |
2:30.64 |
2:36.50 |
5.86 |
| 7 |
Giardina, Amanda G (13)
|
NISEAS |
2:30.97 |
2:38.99 |
8.02 |
| 8 |
McFarland, Emily S (13)
|
NIVICT |
2:48.85 |
2:57.60 |
8.75 |
| 9 |
Welch, Jenna M (15)
|
NIGRSC |
2:52.83 |
2:57.69 |
4.86 |
| 10 |
Visconte, Alexandria L (13)
|
NIFAST |
2:48.45 |
3:00.71 |
12.26 |
| 11 |
Amering, Sophie M (13)
|
NIHAST |
3:13.19 |
3:12.27 |
-0.92 |
|
Doescher, Mackenzi L (15)
|
NIFAST |
2:16.06 |
NS |
- |