| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Williams, Giovanni C (13)
|
NIPACK |
23.51 |
24.25 |
0.74 |
| 2 |
Murray, Maxwell P (15)
|
NISDSC |
25.32 |
24.85 |
-0.47 |
| 3 |
Scaglione, Alexander M (16)
|
NIGCOM |
24.97 |
25.04 |
0.07 |
| 4 |
Ferguson, Aidan J (14)
|
NIMERC |
|
25.45 |
- |
| 5 |
Fixsen, Jordan J (14)
|
NISDSC |
26.80 |
25.96 |
-0.84 |
| 6 |
Cunningham, Dalton M (14)
|
NIGCOM |
27.26 |
26.85 |
-0.41 |
| 7 |
Gilbert, Ajani V (14)
|
NISDSC |
27.24 |
26.95 |
-0.29 |
| 8 |
Liles-McAllister, Kieran J (18)
|
NIMERC |
|
27.17 |
- |
| 9 |
DiFante, Mitchell T (14)
|
NIGCOM |
27.91 |
27.98 |
0.07 |
| 10 |
Falvey, Jacob H (14)
|
NIGCOM |
|
29.00 |
- |