| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Newsome, Amber G (12)
|
NIPMSC |
43.04 |
41.59 |
-1.45 |
| 2 |
Bugbee, Kyla C (12)
|
NIPMSC |
43.74 |
42.91 |
-0.83 |
| 3 |
Stonehouse, Sierra L (12)
|
NIGRSC |
45.34 |
45.11 |
-0.23 |
| 4 |
Zona, Gianna I (12)
|
NIGRSC |
48.94 |
45.17 |
-3.77 |
| 5 |
Rozalski, Jasmine L (12)
|
NIMERC |
47.61 |
46.20 |
-1.41 |
| 6 |
Zerger, Belah (12)
|
NIGRSC |
53.11 |
49.13 |
-3.98 |
| 7 |
White, Shaelyn J (12)
|
NIGCOM |
56.89 |
53.13 |
-3.76 |
| 8 |
Campbell, Brooklyn M (12)
|
NIVICT |
52.59 |
54.28 |
1.69 |
| 9 |
Zeller, Sara L (12)
|
NISDSC |
1:00.36 |
59.58 |
-0.78 |
|
Herron, Ella S (12)
|
NIHAST |
50.92 |
NS |
- |