| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ezenwa, Kelechi (14)
|
IHYS |
28.18 |
28.86 |
0.68 |
| 2 |
Eichinger, Emily C (13)
|
NIGRAY |
28.31 |
29.03 |
0.72 |
| 3 |
Aitchison, Jocelyn C (14)
|
NIGRAY |
29.19 |
29.45 |
0.26 |
| 4 |
Vonica, Camelia S (14)
|
NIGRAY |
|
30.68 |
- |
| 5 |
Curry, Morgan R (13)
|
NIGRAY |
28.86 |
30.80 |
1.94 |
| 6 |
Rocca, Marie (13)
|
NIGRAY |
30.16 |
30.87 |
0.71 |
| 7 |
Soler-Harand, Pauline (13)
|
NIGRAY |
30.27 |
31.06 |
0.79 |
| 8 |
Devine, Mary (14)
|
NIGRAY |
31.55 |
32.39 |
0.84 |
| 9 |
Kuhn, Molly (14)
|
IHYS |
33.73 |
33.71 |
-0.02 |
| 10 |
Adler, Valerie J (14)
|
NIGRAY |
33.38 |
34.42 |
1.04 |
| 11 |
Gupta, Shivika (14)
|
NIGRAY |
40.86 |
36.60 |
-4.26 |