| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
LeFrois-Heath, Emily R (12)
|
NIPACK |
36.73 |
36.26 |
-0.47 |
| 2 |
Markham, Rory P (12)
|
NIFAST |
35.49 |
36.51 |
1.02 |
| 3 |
Rees, Hannah E (12)
|
NIGRSC |
40.20 |
39.91 |
-0.29 |
| 4 |
Cropper, Iala K (12)
|
NIFAST |
39.08 |
40.54 |
1.46 |
| 5 |
Quinmore, Isabelle L (12)
|
NISDSC |
42.96 |
41.20 |
-1.76 |
| 6 |
Kramer, Olivia S (12)
|
NIPACK |
43.62 |
42.40 |
-1.22 |
| 7 |
Baker, Shelby L (12)
|
NIMERC |
41.61 |
43.58 |
1.97 |
| 8 |
Fink, Abigail L (12)
|
NIIRON |
43.41 |
44.54 |
1.13 |
| 9 |
Harris, Saiya'kaede Z (12)
|
NIRRST |
50.78 |
44.55 |
-6.23 |
| 10 |
Williams, Tory L (12)
|
NIPACK |
52.72 |
47.02 |
-5.70 |
| 11 |
Kazmark, Raelyn J (12)
|
NIVICT |
39.13 |
47.65 |
8.52 |
| 12 |
Sotgiu, Isabella V (12)
|
NIFAST |
55.84 |
56.38 |
0.54 |
|
Lopez, Sofia H (12)
|
NIGRSC |
49.10 |
NS |
- |