| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Whitaker, Devyn R (9)
|
NTRACE |
40.63 |
40.55 |
-0.08 |
| 2 |
Vaughn, Kyleigh A (10)
|
NTRACE |
38.53 |
44.18 |
5.65 |
| 3 |
Webb, Kellen D (10)
|
NTRACE |
44.98 |
44.61 |
-0.37 |
| 4 |
Walters, Tenley L (10)
|
NTRACE |
|
44.89 |
- |
| 5 |
Flores, Isabel J (10)
|
NTRACE |
49.46 |
46.32 |
-3.14 |
| 6 |
Gardner, Aelyn I (10)
|
NTRACE |
45.69 |
47.67 |
1.98 |
| 7 |
Harle, Alma C (10)
|
NTRACE |
53.92 |
48.91 |
-5.01 |
| 8 |
Shirley, Kaitlyn C (8)
|
NTRACE |
55.22 |
51.71 |
-3.51 |
| 9 |
Manzo, Macie E (7)
|
NTRACE |
55.37 |
52.07 |
-3.30 |
| 10 |
Gardner, Jade (10)
|
NTRACE |
50.09 |
54.47 |
4.38 |
|
Van Hessen, Isabella G (9)
|
NTRACE |
|
DQ |
- |