| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Huffman, Luke B (15)
|
NTRACE |
52.00 |
48.06 |
-3.94 |
| 2 |
Richardson, Joshua I (17)
|
NTRACE |
48.63 |
48.41 |
-0.22 |
| 3 |
Shim, Abraham J (15)
|
NTRACE |
49.37 |
48.63 |
-0.74 |
| 4 |
Gluck, Ethan S (16)
|
NTRACE |
50.00 |
49.95 |
-0.05 |
| 5 |
Lester, Ryan J (15)
|
NTRACE |
50.40 |
50.35 |
-0.05 |
| 6 |
Esch, Bax D (18)
|
NTRACE |
51.22 |
50.85 |
-0.37 |
| 7 |
English, Addison J (17)
|
NTRACE |
49.91 |
50.95 |
1.04 |
| 8 |
Villarreal, Ricardo A (16)
|
NTRACE |
52.78 |
51.11 |
-1.67 |
| 9 |
Sutton, Caelan G (17)
|
NTRACE |
53.41 |
53.38 |
-0.03 |
| 10 |
Harbin, Alexander P (15)
|
NTRACE |
54.95 |
54.09 |
-0.86 |