| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Whitney, Ella M (10)
|
NTCOR |
2:46.12 |
2:42.97 |
-3.15 |
| 2 |
Souder, Mikayla A (9)
|
NTRACE |
2:54.82 |
2:48.67 |
-6.15 |
| 3 |
Salmassian, Christine K (9)
|
NTDM |
2:56.17 |
2:56.69 |
0.52 |
| 4 |
Portocalis, Brighton W (10)
|
NTDM |
3:20.27 |
3:01.76 |
-18.51 |
| 5 |
Bartlett, Sophia M (9)
|
NTCOR |
3:08.70 |
3:03.69 |
-5.01 |
| 6 |
Chan, Alexa Q (10)
|
NTMTRO |
3:02.94 |
3:04.50 |
1.56 |
| 7 |
Arnold, Brinley G (9)
|
NTSTAR |
3:07.31 |
3:06.41 |
-0.90 |
| 8 |
Boyd, Kaitlyn M (10)
|
NTCOR |
3:19.70 |
3:16.20 |
-3.50 |
| 9 |
Euseppi, Gia L (8)
|
NTDM |
3:37.45 |
3:26.59 |
-10.86 |
|
Dinesh, Renee M (9)
|
NTCOPS |
3:06.62 |
DQ |
- |
|
Valko, Karolina A (9)
|
NTDM |
2:53.89 |
DQ |
- |