| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mitchell, Sophie C (11)
|
NCAQUA |
38.03 |
31.92 |
-6.11 |
| 2 |
Loes, Emily C (12)
|
NCFISH |
59.02 |
32.56 |
-26.46 |
| 3 |
Reeves, Sophia L (12)
|
NCLTNC |
33.06 |
32.59 |
-0.47 |
| 4 |
Walker, Chloe D (12)
|
NCASTF |
30.95 |
33.20 |
2.25 |
| 5 |
Adamkiewicz, Giada R (12)
|
NCWBST |
|
34.31 |
- |
| 6 |
Chabo, Holly L (11)
|
NCAQUA |
|
36.70 |
- |
| 7 |
Hammell, Molly E (11)
|
NCAQUA |
|
37.08 |
- |
| 7 |
Reynolds, Sanaa R (11)
|
NCFISH |
42.78 |
37.08 |
-5.70 |
| 9 |
Williams, Caroline M (11)
|
NCCPDD |
36.70 |
37.35 |
0.65 |
| 10 |
Fipps, Emerson J (12)
|
NCAQUA |
|
38.75 |
- |
| 11 |
Herdman, Anna G (12)
|
NCAQUA |
|
39.05 |
- |
| 12 |
Medina, Mariela M (12)
|
NCFISH |
|
39.12 |
- |
| 13 |
Augustson, Stella (11)
|
NCAQUA |
|
39.44 |
- |
| 14 |
Magdanz, Caitlin M (11)
|
NCLTNC |
51.91 |
41.09 |
-10.82 |
| 15 |
Kimzey, Emma L (11)
|
NCCPDD |
42.12 |
43.62 |
1.50 |
|
Campbell-Godfrey, Jemma J (11)
|
NCBBS |
43.20 |
NS |
- |
|
Choate, Kalia'lani F (12)
|
NCFISH |
35.37 |
NS |
- |