| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Daza Perez, Isabella N (11)
|
NCRMY |
27.49 |
26.91 |
-0.58 |
| 2 |
Packard, Samantha n (13)
|
NCTRY |
26.45 |
27.13 |
0.68 |
| 3 |
Lord, Clara P (15)
|
NCRMY |
27.26 |
27.38 |
0.12 |
| 4 |
Flanagan, Emma R (13)
|
NCNEAL |
28.25 |
27.42 |
-0.83 |
| 5 |
Denton, Elizabeth A (18)
|
NCVACB |
28.06 |
27.95 |
-0.11 |
| 6 |
Eggers, Kaylee J (13)
|
NCRMY |
26.60 |
28.19 |
1.59 |
| 7 |
Forbes, Kayleigh A (16)
|
NCTRY |
28.18 |
28.86 |
0.68 |
| 8 |
Boyd, Jenny K (17)
|
NCVACB |
30.81 |
30.35 |
-0.46 |
| 9 |
Flanagan, Rianne L (13)
|
NCTRY |
30.25 |
31.19 |
0.94 |
| 10 |
Stefanelli, Sierra J (17)
|
NCNEAL |
31.54 |
31.46 |
-0.08 |
| 11 |
Adamkiewicz, Giada R (13)
|
NCWBST |
32.20 |
31.64 |
-0.56 |