| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Beckwith, Molly N (12)
|
NCGATR |
29.80 |
30.36 |
0.56 |
| 2 |
Daza Perez, Isabella N (11)
|
NCRMY |
30.76 |
30.68 |
-0.08 |
| 3 |
Girouard, Madelyn G (11)
|
NCRMY |
35.46 |
35.30 |
-0.16 |
| 4 |
Greene, Brooke E (12)
|
NCTRY |
35.12 |
37.41 |
2.29 |
| 5 |
Bissette, Avery L (12)
|
NCWBST |
50.51 |
38.44 |
-12.07 |
| 6 |
Coulter, Rubygail A (12)
|
NCWBST |
40.09 |
39.85 |
-0.24 |
| 7 |
Flanagan, Madeleine G (12)
|
NCNEAL |
42.93 |
43.65 |
0.72 |
| 8 |
Sharma, Anagha (11)
|
NCWBST |
|
48.16 |
- |
| 9 |
Turner, Tailynn A (11)
|
NCTRY |
|
48.82 |
- |
| 10 |
Council, Mya M (11)
|
NCRMY |
54.73 |
51.53 |
-3.20 |
| 11 |
Boyd, Kassidy L (11)
|
NCVACB |
1:11.84 |
57.90 |
-13.94 |
| 12 |
Honeycutt, Caroline H (11)
|
NCVACB |
55.44 |
1:01.31 |
5.87 |
|
Coates, Nina A (12)
|
NCNEAL |
31.68 |
NS |
- |
|
Greaves, London C (12)
|
NCNEAL |
|
NS |
- |