| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Eggers, Kaylee J (12)
|
NCRMY |
37.51 |
35.56 |
-1.95 |
| 2 |
Reeves, Sophia L (12)
|
NCLTNC |
42.38 |
42.13 |
-0.25 |
| 3 |
Riggs, Skylar R (11)
|
NCLTNC |
46.17 |
43.43 |
-2.74 |
| 4 |
Reynolds, Rebecca L (11)
|
NCASTF |
48.49 |
47.29 |
-1.20 |
| 5 |
Bissette, Avery L (11)
|
NCWBST |
49.31 |
48.13 |
-1.18 |
| 6 |
Hammell, Molly E (11)
|
NCAQUA |
|
48.66 |
- |
| 7 |
Augustson, Stella (11)
|
NCAQUA |
|
49.82 |
- |
| 8 |
Walker, Chloe D (12)
|
NCASTF |
47.05 |
51.54 |
4.49 |
|
Davis, Riley S (11)
|
NCAQUA |
|
NS |
- |
|
Campbell-Godfrey, Jemma J (11)
|
NCBBS |
1:08.03 |
NS |
- |
|
Choate, Kalia'lani F (12)
|
NCFISH |
49.40 |
NS |
- |
|
Magdanz, Caitlin M (11)
|
NCLTNC |
1:13.33 |
NS |
- |