| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jimenez, Isaac N (13)
|
GUUN-T |
|
24.49 |
- |
| 2 |
Fojtik, Ryan D (14)
|
GUPACE |
24.44 |
24.67 |
0.23 |
| 3 |
Daugard, Caden A (13)
|
GUBATS |
25.60 |
24.70 |
-0.90 |
| 4 |
Foo, Yung Hao (13)
|
GUBATS |
24.86 |
24.85 |
-0.01 |
| 5 |
Wilks, Ryan Z (13)
|
GUESCA |
26.40 |
25.25 |
-1.15 |
| 6 |
Pham, Matthew A (13)
|
GUESCA |
26.98 |
26.04 |
-0.94 |
| 7 |
Klabunde, Alexander J (13)
|
GUUN-B |
28.83 |
26.28 |
-2.55 |
| 8 |
Coupland, Coleton D (14)
|
GUPACE |
28.48 |
27.72 |
-0.76 |
| 9 |
Martin, Caleb A (14)
|
GUESCA |
28.01 |
27.75 |
-0.26 |
| 10 |
Bui, Vinh H (14)
|
GUESCA |
31.79 |
29.27 |
-2.52 |
| 11 |
Cox, Everett A (13)
|
GUPLAT |
|
30.71 |
- |
| 12 |
Nguyen, Kelvin T (13)
|
GUESCA |
|
30.89 |
- |
| 13 |
Dang, Hung T (14)
|
GUPLAT |
33.36 |
31.25 |
-2.11 |
| 14 |
Tadlock, Harrison E (14)
|
GUTEST |
31.51 |
31.38 |
-0.13 |
| 15 |
Bui, Long H (13)
|
GUESCA |
31.21 |
31.47 |
0.26 |
| 16 |
Penagos, Christopher P (14)
|
GUESCA |
|
31.58 |
- |
| 17 |
Lindsey, Colin T (13)
|
GUPACE |
38.15 |
34.71 |
-3.44 |
| 18 |
McCormick, Connor K (13)
|
GUBATS |
|
36.12 |
- |
| 19 |
Moore, Sebastian M (13)
|
GUTEST |
|
38.06 |
- |
|
Maldonado, Victor M (13)
|
GUESCA |
31.41 |
NS |
- |
|
Rozo, Juan S (13)
|
GUTEST |
44.64 |
NS |
- |