| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bermudez, Simon (14)
|
PVNCAP |
47.02 |
46.42 |
-0.60 |
| 2 |
Lundgren, Eric C (17)
|
PVNCAP |
48.75 |
48.22 |
-0.53 |
| 3 |
Schmid, JT (15)
|
PVNCAP |
50.58 |
49.62 |
-0.96 |
| 4 |
Ewing, James T (16)
|
PVFISH |
48.72 |
49.83 |
1.11 |
| 5 |
Gorman, Dax P (16)
|
PVNCAP |
54.84 |
52.96 |
-1.88 |
| 6 |
Thliveris, Nicholas J (17)
|
PVNCAP |
54.57 |
53.07 |
-1.50 |
| 7 |
Ly, Nicholas M (16)
|
PVNCAP |
53.61 |
53.42 |
-0.19 |
| 8 |
Cleal, Lilly M (17)
|
PVFISH |
54.26 |
55.14 |
0.88 |
| 9 |
Byrnes, Marco J (16)
|
PVNCAP |
54.55 |
55.71 |
1.16 |
| 10 |
Lewis, Sydney C (15)
|
PVFISH |
57.80 |
58.57 |
0.77 |
| 11 |
Wang, Kathleen (14)
|
PVFISH |
|
59.21 |
- |
|
Park, Gio J (15)
|
PVNCAP |
54.76 |
NS |
- |
|
Sunderhauf, Skylar L (17)
|
PVFISH |
53.49 |
NS |
- |