| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolessar, Madison M (13)
|
MASSC |
27.15 |
26.22 |
-0.93 |
| 2 |
Pujadas, Riley E (13)
|
MASSC |
27.43 |
27.53 |
0.10 |
| 3 |
Diamandi, Michelle I (14)
|
MASSC |
27.74 |
27.57 |
-0.17 |
| 4 |
Egan, Bridget K (13)
|
MASSC |
28.22 |
28.37 |
0.15 |
| 5 |
Hazell, Elsley A (14)
|
MABSC |
28.62 |
28.42 |
-0.20 |
| 6 |
Mandelbaum, Alexandra H (13)
|
MAUNAT |
30.91 |
30.47 |
-0.44 |
| 7 |
Green, Riley H (13)
|
MASSC |
31.79 |
31.23 |
-0.56 |
| 8 |
Sharretts, Emma C (13)
|
MAUNAT |
|
31.52 |
- |
| 9 |
Halbert, Lane C (13)
|
MABSC |
32.63 |
32.01 |
-0.62 |
| 10 |
Torrillo, Nicole L (14)
|
MASSC |
31.49 |
32.48 |
0.99 |
| 11 |
Groover, Lucy B (14)
|
MABSC |
31.50 |
32.93 |
1.43 |
| 12 |
Gale, Morgan A (13)
|
MABSC |
33.35 |
33.42 |
0.07 |
| 13 |
Murphy, Megan R (13)
|
MAUNAT |
37.09 |
34.20 |
-2.89 |
|
Diamandi, Julia A (14)
|
MASSC |
27.40 |
DQ |
- |
|
McGoldrick, Sarah E (14)
|
MABSC |
29.30 |
NS |
- |