| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fuller, Abigail B (17)
|
VAACAC |
23.66 |
24.15 |
0.49 |
| 2 |
McCardle, Grace H (14)
|
VAACAC |
25.57 |
25.60 |
0.03 |
| 3 |
Haise, Sophie A (17)
|
VAACAC |
24.67 |
26.08 |
1.41 |
| 4 |
Kirtley, Miranda B (17)
|
VACCA |
24.95 |
26.29 |
1.34 |
| 5 |
McLaughlin, Delaney L (14)
|
VACCA |
27.43 |
26.55 |
-0.88 |
| 6 |
Samuels, Charlotte G (15)
|
VABASS |
25.75 |
26.56 |
0.81 |
| 7 |
Dojack, Eliah G (15)
|
VAACAC |
26.85 |
27.34 |
0.49 |
| 8 |
Stoner, Ava M (16)
|
VACCA |
26.09 |
27.83 |
1.74 |
| 9 |
Bleecker, Juliana M (17)
|
VACCA |
27.93 |
27.99 |
0.06 |
| 10 |
Hodge, Anna M (13)
|
VACCA |
27.67 |
28.14 |
0.47 |
| 11 |
Hines, Nelle L (14)
|
VABASS |
26.11 |
28.27 |
2.16 |
| 12 |
Conforti-Johnson, Sophia K (13)
|
VACCA |
29.60 |
28.62 |
-0.98 |
| 13 |
Dugas, Georgia K (13)
|
VAACAC |
29.42 |
28.79 |
-0.63 |
| 14 |
McMahon, Natalie E (14)
|
VAVABR |
32.29 |
29.28 |
-3.01 |
| 15 |
Kadela, Ann E (13)
|
VACCA |
30.61 |
29.48 |
-1.13 |
| 16 |
Coleman, Tess H (14)
|
VACCA |
29.11 |
29.66 |
0.55 |
| 17 |
Glick, Olivia R (13)
|
VABASS |
30.97 |
30.98 |
0.01 |
| 18 |
Smith, Saige L (13)
|
VABASS |
31.49 |
31.02 |
-0.47 |
| 19 |
Posadas, Isabella (13)
|
VACCA |
31.71 |
32.92 |
1.21 |
| 20 |
Hodge, Emma C (15)
|
VACCA |
32.45 |
33.10 |
0.65 |
| 21 |
Kovats, Annie C (15)
|
VAVABR |
37.56 |
36.03 |
-1.53 |
| 22 |
Thompson, Heidi (14)
|
VAVABR |
38.99 |
36.35 |
-2.64 |
| 23 |
Carter, Alexa M (13)
|
VACCA |
|
58.15 |
- |
|
Viar, Annaliese R (13)
|
VACCA |
28.74 |
NS |
- |