| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reynoso, Sophia (11)
|
MDHFY |
35.04 |
33.78 |
-1.26 |
| 2 |
Anderson, Trinity A (12)
|
MDHFY |
33.44 |
33.82 |
0.38 |
| 3 |
Rykaczewski, Reagan (11)
|
MASCS |
33.66 |
33.96 |
0.30 |
| 4 |
Ryu, Esther (12)
|
MDHFY |
35.77 |
36.03 |
0.26 |
| 5 |
McDermott, Gabrielle J (11)
|
MDHFY |
45.46 |
38.37 |
-7.09 |
| 6 |
Romanowski, Ashley A (12)
|
MDHFY |
39.08 |
39.50 |
0.42 |
| 7 |
Lewis, Aubrey (12)
|
MASCS |
42.24 |
42.71 |
0.47 |
| 8 |
Kim, Rachel A (11)
|
MDHFY |
45.81 |
44.48 |
-1.33 |
| 9 |
Cole, Winter H (11)
|
MDHFY |
46.19 |
46.60 |
0.41 |
| 10 |
Traore, Jecolia (12)
|
MDHFY |
45.80 |
47.35 |
1.55 |
| 11 |
Clark, Grace (11)
|
MASCS |
48.71 |
49.73 |
1.02 |
| 12 |
King, Zoe (11)
|
MASCS |
1:10.51 |
53.66 |
-16.85 |
| 13 |
Stanley, Harper (12)
|
MASCS |
59.01 |
1:05.57 |
6.56 |
|
Jones, Layla (12)
|
MDHFY |
24.97 |
NS |
- |