| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lineberger, Gracie B (16)
|
NCRACY |
27.44 |
27.96 |
0.52 |
| 2 |
Sechriest, Addyson M (15)
|
NCRACY |
27.63 |
28.06 |
0.43 |
| 3 |
Maiden, Reagan (16)
|
NCRACY |
27.89 |
28.44 |
0.55 |
| 4 |
Sechriest, Makenzie R (17)
|
NCRACY |
28.47 |
29.41 |
0.94 |
| 5 |
Miller, Karis L (15)
|
NCRACY |
30.57 |
29.42 |
-1.15 |
| 6 |
Ginder, Tatiana I (15)
|
NCRACY |
|
31.74 |
- |
| 7 |
Brown, Sachi (15)
|
NCYBAC |
33.54 |
31.98 |
-1.56 |
| 8 |
Elsaid, Retaje H (15)
|
NCRACY |
33.33 |
33.16 |
-0.17 |
| 9 |
Ginder, Maribel R (17)
|
NCRACY |
|
34.97 |
- |
| 10 |
Mobley, Sydney M (17)
|
NCRACY |
38.03 |
37.55 |
-0.48 |
| 11 |
Daugherty, Catherine A (17)
|
NCRACY |
39.21 |
39.24 |
0.03 |
| 12 |
Miller, Shelby R (17)
|
NCRACY |
38.32 |
40.88 |
2.56 |