| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stewart, Sydni R (16)
|
MASAKA |
53.34 |
52.78 |
-0.56 |
| 2 |
Bowne, Jamie O (16)
|
MAGPAC |
53.39 |
54.03 |
0.64 |
| 3 |
Hallam, Kyla M (16)
|
MAGPAC |
|
54.83 |
- |
| 4 |
Wakeley, Laura C (15)
|
MAGPAC |
54.97 |
54.93 |
-0.04 |
| 5 |
Washart, Jordan L (15)
|
MAGPAC |
56.21 |
55.03 |
-1.18 |
| 6 |
Copeland, Brittany M (26)
|
MASAKA |
55.26 |
56.56 |
1.30 |
| 7 |
Parker, Nia C (16)
|
MASAKA |
58.65 |
57.60 |
-1.05 |
| 8 |
Kurtz, Sophia M (16)
|
MAGPAC |
56.27 |
57.90 |
1.63 |
| 9 |
Venit, Veronica (17)
|
MAGPAC |
57.75 |
58.58 |
0.83 |
| 10 |
Bedesem, Caitlin (17)
|
MANRG |
58.36 |
59.93 |
1.57 |
| 11 |
Jackson, India (15)
|
MASAKA |
58.15 |
1:00.88 |
2.73 |
| 12 |
Williams, Gabrielle A (15)
|
MAUN |
|
1:00.92 |
- |
| 13 |
Taylor, Kaia T (15)
|
MASERP |
1:01.91 |
1:03.02 |
1.11 |