| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Han, Malorie M (17)
|
PCTERA |
57.66 |
57.94 |
0.28 |
| 2 |
Fore, Skylar B (16)
|
WIOZ |
56.85 |
58.02 |
1.17 |
| 3 |
House, Emily C (16)
|
PCTERA |
58.00 |
58.03 |
0.03 |
| 4 |
McKernan, Shannon E (18)
|
MAGAAC |
57.14 |
58.12 |
0.98 |
| 5 |
Light, Aislinn J (16)
|
CASCAL |
57.88 |
58.16 |
0.28 |
| 6 |
Krolikowski, Kathryn M (16)
|
CAAZOT |
58.25 |
58.22 |
-0.03 |
| 7 |
Mihok, Kylie A (17)
|
INZSC |
57.32 |
58.57 |
1.25 |
| 8 |
Dunn, Lauren A (17)
|
FLBSS |
59.37 |
59.02 |
-0.35 |
| 9 |
Grinalds, Sarah R (14)
|
CTWRAT |
58.18 |
59.19 |
1.01 |
| 10 |
Bragg, Evie J (16)
|
GADYNA |
59.62 |
59.32 |
-0.30 |
| 11 |
Wells, Katherine V (18)
|
ILSWAQ |
59.06 |
59.53 |
0.47 |
| 12 |
Cooke, Alisa M (17)
|
CACSSC |
58.79 |
59.72 |
0.93 |
| 13 |
Bernal, Aria I (15)
|
GUKATY |
1:01.73 |
1:00.50 |
-1.23 |
| 14 |
Schillinger, Marie-Claire (18)
|
PCWCAB |
1:01.28 |
1:00.72 |
-0.56 |
| 15 |
Yegher, Jaycee M (16)
|
PVRMSC |
1:02.40 |
1:01.13 |
-1.27 |