| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Caimano, Ross M (15)
|
ADGLFY |
53.18 |
53.21 |
0.03 |
| 2 |
Wilkinson, Ryan J (16)
|
ADGLFY |
53.35 |
55.05 |
1.70 |
| 3 |
Waldenmaier, Alexander Z (17)
|
ADGUY |
57.20 |
57.64 |
0.44 |
| 4 |
Dragojevic, Oliver C (16)
|
ADCLIN |
59.72 |
58.52 |
-1.20 |
| 5 |
Romanovsky, Nikita (15)
|
ADSCHE |
59.06 |
59.97 |
0.91 |
| 6 |
Adamo, Joseph J (17)
|
ADUSAM |
51.84 |
1:00.07 |
8.23 |
| 7 |
Napier, Emil C (15)
|
ADUSAM |
1:01.81 |
1:00.19 |
-1.62 |
| 8 |
Silvis, Connor E (15)
|
ADCLIN |
1:01.14 |
1:00.52 |
-0.62 |
| 9 |
Greene, Daniel J (18)
|
ADUSAM |
59.43 |
1:01.62 |
2.19 |
| 10 |
Deets, Devin D (16)
|
ADBENN |
1:18.75 |
1:01.84 |
-16.91 |
| 11 |
Cruz-Figueroa, Eduardo (15)
|
ADSSCY |
1:02.78 |
1:02.85 |
0.07 |
| 12 |
Smalley, Lucas C (17)
|
ADGLFY |
1:03.02 |
1:03.50 |
0.48 |
| 13 |
Parrilla Salgado, Jose J (16)
|
ADCLIN |
1:04.31 |
1:05.37 |
1.06 |
| 14 |
Manny, Eric P (15)
|
ADUSAM |
|
1:14.70 |
- |
|
Rossetti, Derek T (15)
|
ADUSAM |
55.84 |
DQ |
- |
|
Haraden, Brady J (16)
|
ADSCHE |
58.10 |
DQ |
- |
|
Bogdanowicz-Wilson, Lucas C (15)
|
ADSCHE |
51.67 |
NS |
- |
|
Coughlan, Gavin G (18)
|
ADGLFY |
54.06 |
NS |
- |