| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
June, Mya G (16)
|
ADJJSC |
26.10 |
26.78 |
0.68 |
| 2 |
Valerian, Caitie G (15)
|
ADDELM |
26.68 |
27.06 |
0.38 |
| 3 |
Wang, Catherine J (15)
|
ADSCHE |
27.53 |
27.58 |
0.05 |
| 4 |
Lupe, Gabrielle (16)
|
ADJJSC |
27.41 |
27.80 |
0.39 |
| 5 |
Drummond, Jenna D (16)
|
ADUN |
25.98 |
27.90 |
1.92 |
| 6 |
Phelps, Sara M (17)
|
ADBSPA |
27.07 |
28.26 |
1.19 |
| 7 |
Phelps, Megan K (17)
|
ADBSPA |
27.05 |
28.33 |
1.28 |
| 8 |
Jerabek, Ulka G (15)
|
ADDELM |
27.57 |
28.41 |
0.84 |
| 9 |
Phillips, Mckenna L (15)
|
ADBSPA |
27.30 |
28.45 |
1.15 |
| 10 |
Gayton, Kailey C (15)
|
ADGLFY |
27.34 |
28.52 |
1.18 |
| 11 |
Felton, Emily R (16)
|
ADGLFY |
27.14 |
28.62 |
1.48 |
| 12 |
Bartlett, Carys E (16)
|
ADSCHE |
28.21 |
28.66 |
0.45 |
| 13 |
Cox, Madeline P (15)
|
ADGRGF |
29.20 |
29.28 |
0.08 |
| 14 |
Leaver, Madison M (15)
|
ADGRGF |
30.98 |
30.66 |
-0.32 |
| 15 |
Miller, Alexandria M (17)
|
ADBSPA |
29.19 |
30.74 |
1.55 |
| 16 |
Welch, Victoria L (15)
|
ADJJSC |
30.25 |
31.13 |
0.88 |
| 17 |
Stevenson, MacKenzi K (17)
|
ADGUY |
29.92 |
31.65 |
1.73 |
| 18 |
McCurry, Alexa M (15)
|
ADGRGF |
31.98 |
31.68 |
-0.30 |
|
Marion, Perrin L (15)
|
ADBENN |
27.33 |
NS |
- |
|
Asciutto, Emily M (16)
|
ADBENN |
27.61 |
NS |
- |