| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Valerian, Caitie G (15)
|
ADDELM |
1:05.65 |
1:06.87 |
1.22 |
| 2 |
June, Mya G (16)
|
ADJJSC |
1:04.76 |
1:07.74 |
2.98 |
| 3 |
Wang, Catherine J (15)
|
ADSCHE |
1:06.87 |
1:08.69 |
1.82 |
| 4 |
Russo, Olivia G (16)
|
ADCLIN |
1:09.83 |
1:10.07 |
0.24 |
| 5 |
Giumarra, Natalie A (17)
|
ADGLFY |
1:06.02 |
1:12.03 |
6.01 |
| 6 |
Drummond, Jenna D (16)
|
ADUN |
1:06.20 |
1:12.45 |
6.25 |
| 7 |
Brennen, Riley E (15)
|
ADCLIN |
1:09.12 |
1:13.19 |
4.07 |
| 8 |
Stevenson, MacKenzi K (17)
|
ADGUY |
1:10.47 |
1:14.92 |
4.45 |
| 9 |
Miller, Alexandria M (17)
|
ADBSPA |
1:15.83 |
1:17.21 |
1.38 |
| 10 |
Harmon, Anna E (15)
|
ADSARY |
|
1:17.29 |
- |