| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blaum, Sarah T (8)
|
ADBSPA |
36.89 |
36.91 |
0.02 |
| 2 |
Ruscio, Emily A (8)
|
ADSCHE |
39.56 |
37.02 |
-2.54 |
| 3 |
Rogowski, Isabella S (8)
|
ADSCHE |
39.90 |
39.10 |
-0.80 |
| 4 |
Relyea, Cadence G (8)
|
ADDELM |
44.77 |
39.57 |
-5.20 |
| 5 |
Stewart Fraser, Thornton R (8)
|
ADALBS |
41.36 |
39.58 |
-1.78 |
| 6 |
Sancilio, Elizabeth G (8)
|
ADDELM |
|
40.04 |
- |
| 7 |
Cao, Aimee W (8)
|
ADJJSC |
42.76 |
41.64 |
-1.12 |
| 8 |
Salvi, Riley (8)
|
ADJJSC |
58.94 |
43.75 |
-15.19 |
| 9 |
Breckenridge, Lucy F (8)
|
ADSCHE |
45.88 |
43.86 |
-2.02 |
| 10 |
Waite, Cadence (7)
|
ADJJSC |
48.68 |
45.01 |
-3.67 |
| 11 |
Kim, Catherine (7)
|
ADSCHE |
51.69 |
45.77 |
-5.92 |
| 12 |
Shukla, Aarya O (8)
|
ADSCHE |
48.44 |
46.14 |
-2.30 |
| 13 |
Blaum, Kathryn A (8)
|
ADBSPA |
46.64 |
48.22 |
1.58 |
| 14 |
Panetta, Skylar J (7)
|
ADSCHE |
57.14 |
48.82 |
-8.32 |
| 15 |
Polomsky, Alexandra E (7)
|
ADDELM |
|
49.54 |
- |
| 16 |
Jacobi, Clara S (8)
|
ADBSPA |
53.03 |
51.55 |
-1.48 |
| 17 |
Ruff, Mikaella K (7)
|
ADALBS |
54.08 |
53.05 |
-1.03 |
| 18 |
Robusto, Mia M (8)
|
ADBSPA |
1:03.07 |
58.77 |
-4.30 |
| 19 |
Lofink, Ella F (6)
|
ADSCHE |
1:00.76 |
59.35 |
-1.41 |
| 20 |
Doyle, Havah M (7)
|
ADSARY |
1:00.88 |
59.88 |
-1.00 |
| 21 |
Jenkins, Caroline J (6)
|
ADBSPA |
|
1:01.49 |
- |
| 22 |
Wilson, Anna M (8)
|
ADBSPA |
1:14.12 |
1:02.66 |
-11.46 |
| 23 |
Larsen, Olivia (7)
|
ADJJSC |
1:02.16 |
1:04.50 |
2.34 |
| 24 |
Perez, Eva L (6)
|
ADBFIN |
1:03.68 |
1:05.96 |
2.28 |
| 25 |
Hou, Irena Y (6)
|
ADDELM |
|
1:09.90 |
- |
| 26 |
Lyons, Avery G (6)
|
ADBFIN |
1:20.21 |
1:10.16 |
-10.05 |
| 27 |
Basile, Robin J (6)
|
ADALBS |
1:05.83 |
1:10.52 |
4.69 |
| 28 |
Gosson, Mickenzi L (7)
|
ADBSPA |
|
1:25.63 |
- |
|
Carstens, Elia L (6)
|
ADBSPA |
|
DQ |
- |
|
Fountain-Roesser, Jade M (8)
|
ADDELM |
52.39 |
NS |
- |
|
Cahill, Faye M (8)
|
ADDELM |
|
NS |
- |
|
Waldman, Jacqueline C (8)
|
ADUN |
41.14 |
NS |
- |