| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fraley, Ella R (12)
|
ADBFIN |
27.85 |
27.94 |
0.09 |
| 2 |
Blaum, Sarah T (11)
|
ADSCHE |
28.51 |
28.52 |
0.01 |
| 3 |
Streeter, Olivia E (12)
|
ADSCHE |
27.63 |
28.67 |
1.04 |
| 4 |
Johnston, Layla A (12)
|
ADSCHE |
28.96 |
29.19 |
0.23 |
| 5 |
Basile, Isabella M (12)
|
ADSCHE |
29.81 |
29.87 |
0.06 |
| 6 |
Dunbar, May R (12)
|
ADUSAM |
31.09 |
32.09 |
1.00 |
| 7 |
Taylor, Natalie (11)
|
ADSCHE |
32.89 |
33.02 |
0.13 |
| 8 |
Breckenridge, Lucy F (11)
|
ADSCHE |
33.37 |
33.04 |
-0.33 |
| 9 |
Ruscio, Emily A (11)
|
ADSCHE |
35.20 |
33.18 |
-2.02 |
| 10 |
Miron, Sofia L (12)
|
ADBFIN |
32.13 |
33.35 |
1.22 |
| 11 |
Chen, Serena (12)
|
ADBFIN |
34.82 |
34.65 |
-0.17 |
| 12 |
Muldner, Juliana G (11)
|
ADSCHE |
34.44 |
34.72 |
0.28 |
| 13 |
Durfee, Emily G (11)
|
ADSCHE |
35.81 |
34.76 |
-1.05 |
| 14 |
Martone, Molly D (12)
|
ADSCHE |
37.77 |
34.79 |
-2.98 |
| 15 |
Puls, Kiera A (11)
|
ADBFIN |
34.00 |
34.95 |
0.95 |
| 16 |
Toomajian, Stella T (11)
|
ADUSAM |
39.17 |
36.47 |
-2.70 |
| 17 |
Ventura, Colbie A (12)
|
ADSCHE |
37.07 |
37.26 |
0.19 |
| 18 |
Romanovsky, Lili (12)
|
ADSCHE |
40.05 |
37.34 |
-2.71 |
| 19 |
Griffin, Alexis M (12)
|
ADSCHE |
41.13 |
38.70 |
-2.43 |
| 20 |
Aldridge, Linnea G (11)
|
ADSCHE |
38.80 |
38.72 |
-0.08 |
| 21 |
Dollar, Brieanna N (12)
|
ADSCHE |
38.41 |
39.19 |
0.78 |
| 22 |
Li, Naomi L (12)
|
ADBFIN |
|
39.35 |
- |
| 23 |
Schlansker, Bowe V (11)
|
ADSCHE |
42.28 |
39.92 |
-2.36 |
| 24 |
Dansie, Stella J (11)
|
ADSCHE |
40.46 |
40.27 |
-0.19 |
| 25 |
Applebaum, Aaliyah R (11)
|
ADCANA |
44.58 |
43.56 |
-1.02 |
| 26 |
Lefco, Elena T (12)
|
ADSCHE |
45.95 |
45.89 |
-0.06 |
| 27 |
Donato, Alexandra C (11)
|
ADSCHE |
48.38 |
46.72 |
-1.66 |
| 28 |
Obrien, Gianna R (12)
|
ADSCHE |
50.54 |
46.87 |
-3.67 |
| 29 |
Cook, Abigail R (12)
|
ADSCHE |
|
48.15 |
- |
| 30 |
Wilson, Anna M (11)
|
ADSCHE |
48.07 |
49.96 |
1.89 |
|
Rakoske, Morgan M (11)
|
ADSCHE |
45.10 |
NS |
- |
|
Kim, Grace (12)
|
ADSCHE |
34.69 |
NS |
- |