| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Blaum, Sarah T (12)
|
ADSCHE |
28.99 |
29.71 |
0.72 |
| 2 |
Dheeradhada, Arya J (12)
|
ADALBS |
31.84 |
31.62 |
-0.22 |
| 3 |
Weil, Cadhla L (12)
|
ADSCHE |
36.74 |
32.01 |
-4.73 |
| 4 |
Adams, Michelle E (11)
|
ADCPP |
30.86 |
32.56 |
1.70 |
| 5 |
Kim, Catherine (11)
|
ADSCHE |
34.88 |
35.07 |
0.19 |
| 6 |
Cunningham, Isabelle G (12)
|
ADCPP |
40.03 |
35.81 |
-4.22 |
| 7 |
Rio, Willa K (12)
|
ADNHA |
36.90 |
36.36 |
-0.54 |
| 8 |
Laurie, Makena G (11)
|
ADHAWK |
36.45 |
36.40 |
-0.05 |
| 9 |
Attia, Laila M (11)
|
ADSCHE |
39.48 |
36.41 |
-3.07 |
| 10 |
Taylor, Natalie (11)
|
ADSCHE |
35.63 |
36.74 |
1.11 |
| 11 |
Padula, Emma R (11)
|
ADDELM |
36.63 |
37.07 |
0.44 |
| 12 |
Relyea, Cadence G (12)
|
ADDELM |
35.48 |
37.29 |
1.81 |
| 13 |
MacLeod, Addison M (11)
|
ADUSAM |
35.61 |
37.47 |
1.86 |
| 14 |
Cho, Anna Y (12)
|
ADALBS |
40.11 |
37.56 |
-2.55 |
| 15 |
Miron, Sofia L (12)
|
ADBFIN |
33.06 |
37.57 |
4.51 |
| 16 |
Ness, Elsie K (12)
|
ADSCHE |
38.09 |
37.75 |
-0.34 |
| 17 |
Allinson, Jane M (11)
|
ADDELM |
39.09 |
37.76 |
-1.33 |
| 18 |
Walmsley, Lauren M (11)
|
ADCPP |
37.43 |
39.21 |
1.78 |
| 19 |
Cosh, Mallory G (11)
|
ADSCHE |
40.16 |
39.23 |
-0.93 |
| 20 |
May, Erin (12)
|
ADDELM |
47.04 |
40.57 |
-6.47 |
| 21 |
Toomajian, Stella T (12)
|
ADUSAM |
|
43.60 |
- |
| 22 |
Quackenbush, Leah H (11)
|
ADALBS |
39.95 |
45.00 |
5.05 |
| 23 |
Shifrin, Yeva R (11)
|
ADNHA |
42.00 |
45.01 |
3.01 |
| 24 |
Zaderej, Sophia V (11)
|
ADCPP |
|
45.29 |
- |
| 25 |
Miller, Isabella M (11)
|
ADCPP |
1:01.11 |
46.59 |
-14.52 |
| 26 |
Mastri, Emilia N (12)
|
ADSCHE |
|
47.67 |
- |
| 27 |
Luley, Mya E (12)
|
ADNHA |
56.44 |
52.45 |
-3.99 |
|
Walker, Aspen F (12)
|
ADALBS |
|
DQ |
- |
|
Sancilio, Elizabeth G (12)
|
ADDELM |
35.02 |
DQ |
- |
|
Barry, Cameron E (12)
|
ADUSAM |
|
NS |
- |