| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sundgren, Alexa K (16)
|
AMANSC |
2:11.32 |
2:16.62 |
5.30 |
| 2 |
Kennedy, Delaney S (17)
|
AMANSC |
2:24.56 |
2:24.59 |
0.03 |
| 3 |
Baiano, Gabriella L (17)
|
AMSTEE |
2:25.27 |
2:25.03 |
-0.24 |
| 4 |
Stratigos, Melina T (15)
|
AMPEAQ |
2:27.51 |
2:31.79 |
4.28 |
| 5 |
Wasilco, Madison L (15)
|
AMANSC |
2:36.28 |
2:33.14 |
-3.14 |
| 6 |
Kwiatkowski, Megan R (17)
|
AMPTSC |
2:21.92 |
2:34.24 |
12.32 |
| 7 |
Walker, Julie A (16)
|
AMSTEE |
2:36.30 |
2:34.68 |
-1.62 |
| 8 |
Lange, Paige M (15)
|
AMNHA |
2:38.73 |
2:41.57 |
2.84 |
| 9 |
Callaghan, Seraphina N (16)
|
AM3RVS |
2:35.00 |
2:42.48 |
7.48 |
| 10 |
Dunn, Riley N (16)
|
AMPEAQ |
2:49.73 |
2:44.12 |
-5.61 |
| 11 |
Hayson, Jenna N (17)
|
AM3RVS |
2:41.31 |
2:46.72 |
5.41 |
| 12 |
Bixler, Willow T (16)
|
AMFAST |
2:37.73 |
2:47.29 |
9.56 |
| 13 |
Poniatowski, Shelby A (15)
|
AMNHA |
2:54.74 |
2:56.23 |
1.49 |