| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sundgren, Alexa K (16)
|
AMANSC |
29.08 |
28.77 |
-0.31 |
| 2 |
Kennedy, Delaney S (17)
|
AMANSC |
30.36 |
30.16 |
-0.20 |
| 3 |
Roman, Stephanie M (16)
|
AMSTEE |
30.19 |
30.91 |
0.72 |
| 4 |
Stratigos, Melina T (15)
|
AMPEAQ |
30.47 |
31.05 |
0.58 |
| 5 |
Hayson, Jenna N (17)
|
AM3RVS |
32.64 |
31.32 |
-1.32 |
| 6 |
Baiano, Gabriella L (17)
|
AMSTEE |
30.55 |
31.39 |
0.84 |
| 7 |
Lange, Paige M (15)
|
AMNHA |
31.23 |
31.65 |
0.42 |
| 8 |
Dube, Madeline K (15)
|
AMNHA |
32.36 |
31.85 |
-0.51 |
| 9 |
Wasilco, Madison L (15)
|
AMANSC |
31.46 |
32.29 |
0.83 |
| 10 |
Kwiatkowski, Megan R (17)
|
AMPTSC |
30.84 |
33.79 |
2.95 |
| 11 |
Callaghan, Seraphina N (16)
|
AM3RVS |
37.00 |
33.86 |
-3.14 |
| 12 |
Mariner, Ava B (15)
|
AMPTSC |
32.32 |
34.16 |
1.84 |
| 13 |
Poniatowski, Shelby A (15)
|
AMNHA |
36.90 |
35.71 |
-1.19 |
| 14 |
Bixler, Willow T (16)
|
AMFAST |
34.20 |
36.27 |
2.07 |
| 15 |
Dunn, Riley N (16)
|
AMPEAQ |
35.45 |
36.73 |
1.28 |
|
Huwar, Sophia G (16)
|
AMANSC |
29.66 |
NS |
- |