| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Larsen, Sarah E (9)
|
MRLIAC |
|
2:45.77 |
- |
| 2 |
Fleck, Kristina S (9)
|
PVNCAP |
3:08.68 |
2:46.29 |
-22.39 |
| 3 |
Chun, April S (9)
|
MRLIAC |
3:04.12 |
2:48.24 |
-15.88 |
| 4 |
Bartholomew, Isla (9)
|
PVRMSC |
2:59.30 |
2:49.21 |
-10.09 |
| 5 |
Phillips, Alexandra J (9)
|
MADST |
3:09.56 |
2:53.42 |
-16.14 |
| 6 |
Kuhlen, Kate R (9)
|
VANOVA |
3:12.35 |
2:55.61 |
-16.74 |
| 7 |
Caiazza-Blum, Alice Y (9)
|
PVRMSC |
3:02.07 |
2:58.35 |
-3.72 |
| 8 |
Webb, Maria D (9)
|
PVRMSC |
3:07.20 |
3:01.14 |
-6.06 |
| 9 |
Flinn, Amie J (9)
|
PVNCAP |
3:12.20 |
3:03.57 |
-8.63 |
| 10 |
Hotem, Kaitlyn R (9)
|
VANOVA |
3:16.17 |
3:04.10 |
-12.07 |
| 11 |
Cypher, Ella M (9)
|
PVNCAP |
3:10.72 |
3:05.06 |
-5.66 |
| 12 |
Jessup, Abigail M (9)
|
VANOVA |
3:07.49 |
3:05.29 |
-2.20 |
| 13 |
DeBaecke, Emerson G (9)
|
MADST |
4:26.82 |
3:06.68 |
-80.14 |
| 14 |
Nam, Lauren (9)
|
MRLIAC |
|
3:14.18 |
- |