| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Colborn, Georgia A (12)
|
MADST |
55.74 |
54.01 |
-1.73 |
| 2 |
Qin, Amy W (12)
|
PVRMSC |
55.39 |
54.36 |
-1.03 |
| 3 |
Fanelli, Lila R (12)
|
MADST |
1:00.81 |
57.67 |
-3.14 |
| 4 |
Ruszala, Hailey C (12)
|
MADST |
58.48 |
58.00 |
-0.48 |
| 5 |
Rigas, Christina E (12)
|
MADST |
59.64 |
58.44 |
-1.20 |
| 6 |
Crane, Zoe A (12)
|
PVRMSC |
1:00.19 |
58.69 |
-1.50 |
| 7 |
Phillips, Reagan E (12)
|
PVRMSC |
59.04 |
58.75 |
-0.29 |
| 8 |
Brady, Peyton S (12)
|
PVOCCS |
1:00.41 |
59.45 |
-0.96 |
| 9 |
Abouraya, Ruqayyah M (12)
|
PVRMSC |
1:00.62 |
1:00.37 |
-0.25 |
| 10 |
Von Herbulis, Lillian M (12)
|
PVOCCS |
1:03.15 |
1:01.22 |
-1.93 |
| 11 |
Worth, Brooke M (12)
|
PVOCCS |
1:01.75 |
1:01.33 |
-0.42 |
| 12 |
Hannam, Charlotte Q (12)
|
PVOCCS |
1:01.58 |
1:02.80 |
1.22 |