| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bacon, Phoebe M (14)
|
PVNCAP |
24.14 |
24.16 |
0.02 |
| 2 |
Fischer, Maya (14)
|
PVNCAP |
24.24 |
24.49 |
0.25 |
| 3 |
Warner, Bella N (14)
|
MADST |
24.42 |
24.67 |
0.25 |
| 4 |
Landeryou, Sophia A (14)
|
PVNCAP |
24.50 |
24.71 |
0.21 |
| 5 |
LeFauve, Jolie E (14)
|
PVRMSC |
24.70 |
25.01 |
0.31 |
| 6 |
Mara, Madeline A (14)
|
PVRMSC |
25.01 |
25.14 |
0.13 |
| 7 |
Paoletti, Isabella N (14)
|
MADST |
24.75 |
25.64 |
0.89 |
| 8 |
Elliott, Sarah E (14)
|
PVRMSC |
25.63 |
25.80 |
0.17 |
| 9 |
Bunting, Abigail B (14)
|
VANOVA |
25.41 |
25.94 |
0.53 |
| 10 |
Competello, Madeline G (14)
|
MRLIAC |
25.11 |
25.95 |
0.84 |
| 11 |
Isley, Courtney A (14)
|
VANOVA |
25.38 |
26.45 |
1.07 |
| 12 |
Hong, Katie (14)
|
MRLIAC |
26.85 |
26.61 |
-0.24 |
| 13 |
Thomas, Avery L (14)
|
MADST |
27.21 |
27.02 |
-0.19 |
| 14 |
Nowak, Nicole (14)
|
MRLIAC |
27.79 |
28.36 |
0.57 |
|
Burton, Morgan T (14)
|
VAUN |
24.75 |
25.41 |
0.66 |