| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Donoghue, Rowan B (10)
|
MADST |
27.70 |
27.73 |
0.03 |
| 2 |
Dworak, Andrea M (10)
|
PVRMSC |
28.31 |
28.05 |
-0.26 |
| 3 |
Wu, Jingjing (10)
|
PVRMSC |
29.48 |
28.46 |
-1.02 |
| 4 |
Stabler, Ainsley (10)
|
PVOCCS |
29.71 |
29.44 |
-0.27 |
| 5 |
Picardo, Nina Karylle B (10)
|
PVOCCS |
29.87 |
29.51 |
-0.36 |
| 6 |
Caiazza-Blum, Alice Y (10)
|
PVRMSC |
30.32 |
30.10 |
-0.22 |
| 7 |
Ingram, Claire O (10)
|
MDMAC |
31.22 |
30.36 |
-0.86 |
| 8 |
DeBaecke, Emerson G (10)
|
MADST |
30.51 |
30.58 |
0.07 |
| 9 |
Phillips, Alexandra J (10)
|
MADST |
30.12 |
30.61 |
0.49 |
| 9 |
Zalalutdinov, Darya H (10)
|
PVRMSC |
31.32 |
30.61 |
-0.71 |
| 11 |
Luetkenhaus, Caroline E (10)
|
PVOCCS |
30.80 |
30.78 |
-0.02 |
| 12 |
Smariga, Anya (10)
|
MDMAC |
33.60 |
31.24 |
-2.36 |
| 13 |
Reen, Riley R (10)
|
MDMAC |
31.92 |
31.76 |
-0.16 |
| 14 |
Oliver, Sarah A (10)
|
PVOCCS |
32.71 |
33.27 |
0.56 |
| 15 |
Thornett, Emily E (10)
|
MDMAC |
35.55 |
33.92 |
-1.63 |