| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hill, Gennaro W (10)
|
NIAYST |
42.31 |
41.05 |
-1.26 |
| 2 |
Martin, Charles M (9)
|
ADONEI |
42.72 |
41.13 |
-1.59 |
| 3 |
Olmsted, Luke (10)
|
NISKAN |
44.36 |
45.98 |
1.62 |
| 4 |
Austin, William E (10)
|
NISKAN |
47.94 |
47.39 |
-0.55 |
| 5 |
Echols, Ethan _ (9)
|
NISKAN |
50.40 |
47.79 |
-2.61 |
| 6 |
Urf, Trey (10)
|
ADWAT |
48.07 |
48.36 |
0.29 |
| 7 |
Wilson, Carter S (10)
|
NIAYST |
50.81 |
48.56 |
-2.25 |
| 8 |
Temming, Ethan (10)
|
ADONFY |
48.82 |
48.91 |
0.09 |
| 9 |
Van Kersbergen, Jensen R (9)
|
NISKAN |
49.54 |
50.09 |
0.55 |
| 10 |
Underwood, Xander A (10)
|
NICORY |
53.17 |
51.91 |
-1.26 |
| 11 |
Charlebois, Henry (9)
|
ADWAT |
57.53 |
54.73 |
-2.80 |
| 12 |
Abrams, Collin J (10)
|
ADONEI |
57.68 |
57.80 |
0.12 |
| 13 |
Johnson, Lucas T (10)
|
NICORY |
1:04.13 |
1:01.34 |
-2.79 |
| 14 |
Wallace, Matt (9)
|
ADWAT |
57.37 |
1:02.54 |
5.17 |
| 15 |
Boomhower, Alexander (10)
|
ADWAT |
59.94 |
1:05.45 |
5.51 |