| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Prushinski, Alison C (18)
|
MAUN |
24.41 |
25.90 |
1.49 |
| 2 |
Corcoran, Victoria (16)
|
MRDVWM |
26.27 |
26.90 |
0.63 |
| 3 |
Brock, Abigail J (16)
|
MAUN |
26.51 |
27.09 |
0.58 |
| 4 |
Mohyla, Aislynn J (17)
|
MRAGMV |
27.09 |
27.53 |
0.44 |
| 5 |
Hartmann, Sierra (15)
|
MAPFY |
26.16 |
27.79 |
1.63 |
| 6 |
Prushinski, Erica (16)
|
MAUN |
26.47 |
27.92 |
1.45 |
| 7 |
Kawash, Molly K (15)
|
MAUN |
29.06 |
29.32 |
0.26 |
| 8 |
Wright, Hannah E (15)
|
MRNYSA |
27.40 |
29.52 |
2.12 |
| 9 |
Sommer, Stefanie M (15)
|
MRAGMV |
28.51 |
29.54 |
1.03 |
| 10 |
Staley, Sofia R (15)
|
MRAGMV |
29.40 |
29.74 |
0.34 |
| 11 |
Dunn, Emily (15)
|
MAUN |
30.51 |
32.14 |
1.63 |
| 12 |
Bonavoglia, Savannah (15)
|
MAUN |
|
32.70 |
- |
| 13 |
Ring, Lydia E (15)
|
MRWSSC |
30.20 |
33.41 |
3.21 |
| 14 |
DiPisa, Bella (16)
|
MAUN |
33.29 |
34.16 |
0.87 |
| 15 |
Nanartowicz, Emily E (15)
|
MRAGMV |
37.89 |
36.40 |
-1.49 |
| 16 |
Henry, Shataiya D (15)
|
MRYBAR |
41.35 |
48.85 |
7.50 |