| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Samson, Reaghan (12)
|
CTRAYS |
28.34 |
28.31 |
-0.03 |
| 2 |
Jackson, Jaeda (11)
|
CTGWYB |
34.66 |
33.68 |
-0.98 |
| 3 |
Shabani, Arsa (11)
|
CTGWYB |
35.24 |
35.83 |
0.59 |
| 4 |
DellaVecchia, Katelyn R (12)
|
CTRAYS |
37.44 |
36.19 |
-1.25 |
| 5 |
Baribault, Jamie E (12)
|
CTRAYS |
37.79 |
38.26 |
0.47 |
| 6 |
Aggarwal, Preeti (12)
|
CTGWYB |
33.11 |
40.75 |
7.64 |
| 7 |
Irazabal, Olivia M (11)
|
CTRAYS |
|
41.13 |
- |
| 8 |
Eggers, Alexandria N (11)
|
CTRAYS |
43.25 |
41.15 |
-2.10 |
| 9 |
Cieslik, Amelia E (12)
|
CTRAYS |
46.73 |
42.52 |
-4.21 |
| 10 |
Nattrass, Elizabeth L (11)
|
CTRAYS |
|
1:01.07 |
- |