| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gashi, Deona (10)
|
CTGWYB |
41.54 |
42.44 |
0.90 |
| 2 |
Graham, Jayda (12)
|
CTGWYB |
41.31 |
42.54 |
1.23 |
| 3 |
Burdeshaw, Raegan E (10)
|
CTRAYS |
44.08 |
42.73 |
-1.35 |
| 4 |
Alejandro, Ruth C (12)
|
CTRAYS |
45.86 |
43.38 |
-2.48 |
| 5 |
Stead, Molly (12)
|
CTGWYB |
|
48.29 |
- |
| 6 |
DellaVecchia, Katelyn R (12)
|
CTRAYS |
50.00 |
48.52 |
-1.48 |
| 7 |
Patrick, Jahnii (10)
|
CTGWYB |
53.13 |
48.99 |
-4.14 |
| 8 |
Shabani, Arsa (11)
|
CTGWYB |
48.88 |
50.88 |
2.00 |
| 9 |
Kennedy, Mia A (11)
|
CTRAYS |
49.32 |
51.28 |
1.96 |
| 10 |
Pugliese, Madelyn (11)
|
CTRAYS |
54.12 |
53.08 |
-1.04 |
| 11 |
Baribault, Jamie E (12)
|
CTRAYS |
57.49 |
1:00.59 |
3.10 |
| 12 |
Scott, Brooke H (9)
|
CTRAYS |
1:01.71 |
1:03.09 |
1.38 |
| 13 |
Nattrass, Elizabeth L (11)
|
CTRAYS |
|
1:08.91 |
- |
|
Weirshousky, Violette (10)
|
CTGWYB |
58.43 |
DQ |
- |