| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Delehanty, Aidan W (14)
|
NIRRST |
27.64 |
24.01 |
-3.63 |
| 1 |
Pierce, Talyn E (16)
|
NIVICT |
23.75 |
24.01 |
0.26 |
| 3 |
Portwood, Yushi T (14)
|
NIHAWK |
24.76 |
25.20 |
0.44 |
| 4 |
Palumbo, Domenic D (15)
|
NIHAWK |
26.11 |
25.32 |
-0.79 |
| 5 |
Gillis, Ian T (16)
|
NIGRSC |
28.94 |
25.69 |
-3.25 |
| 6 |
LaMere, Alexander E (15)
|
NIGRSC |
|
26.18 |
- |
| 7 |
Bronson, Dylan T (15)
|
NISDSC |
25.82 |
26.42 |
0.60 |
| 8 |
Bay, Jackson K (13)
|
NIVICT |
28.05 |
26.92 |
-1.13 |
| 9 |
Koralewski, Mitchell K (15)
|
NIHAWK |
28.78 |
27.98 |
-0.80 |
| 10 |
Wu, Andrew (14)
|
NIGCOM |
28.81 |
28.84 |
0.03 |
| 11 |
Werth, Ethan E (13)
|
NIGRSC |
|
30.26 |
- |
| 12 |
Ciravola, Jayden T (14)
|
NIHAST |
29.95 |
31.21 |
1.26 |
| 13 |
Peters, Ryan R (14)
|
NIGCOM |
37.59 |
36.43 |
-1.16 |
|
Palacio, Gabriel D (15)
|
NIHAWK |
26.71 |
NS |
- |
|
Palacio, Raphael D (15)
|
NIHAWK |
24.80 |
NS |
- |
|
Gargano, Alexander F (17)
|
NIHAST |
|
NS |
- |