| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Geary, Kaitlyn J (10)
|
NIMERC |
49.41 |
45.78 |
-3.63 |
| 2 |
Richards, Sydney R (9)
|
NIGRAT |
49.12 |
47.71 |
-1.41 |
| 3 |
Kelley, Michaela G (10)
|
NIUN |
50.41 |
48.78 |
-1.63 |
| 4 |
Young, Jazmine J (10)
|
NIGRSC |
|
49.12 |
- |
| 5 |
Share, Elizabeth A (10)
|
NIPMSC |
46.65 |
50.46 |
3.81 |
| 6 |
Santmyer, Grace E (10)
|
NISDSC |
53.54 |
52.28 |
-1.26 |
| 7 |
Ashline, Grace E (10)
|
NISDSC |
55.67 |
52.39 |
-3.28 |
| 8 |
Siciliano, Paige E (10)
|
NIGRSC |
54.09 |
53.08 |
-1.01 |
| 9 |
Kazmark, Olivia N (10)
|
NIVICT |
1:13.09 |
53.17 |
-19.92 |
| 10 |
Robinson, Sara C (10)
|
NISDSC |
56.69 |
53.52 |
-3.17 |
| 11 |
Richards, Kylie G (10)
|
NIHAWK |
|
57.57 |
- |
| 12 |
Nguessamble, Tristyn D (10)
|
NISDSC |
1:01.45 |
58.01 |
-3.44 |
| 13 |
Kenny, Elisabeth A (10)
|
NIUN |
1:02.35 |
58.06 |
-4.29 |
| 14 |
Schwandt, Natalie K (9)
|
NIGRAT |
1:05.21 |
59.99 |
-5.22 |
| 15 |
Munson, Sophie M (9)
|
NIGRSC |
|
1:00.13 |
- |
| 16 |
Smith, Lindsey A (10)
|
NIGRSC |
1:05.28 |
1:00.59 |
-4.69 |
| 17 |
Olbrys, Lilja (10)
|
NIVICT |
58.08 |
1:03.15 |
5.07 |
| 18 |
Greisch, Alisha D (9)
|
NIGRSC |
1:05.06 |
1:03.23 |
-1.83 |
| 19 |
Kapral, Chloe H (10)
|
NIHAWK |
|
1:04.40 |
- |
| 20 |
Rioux, Alexis E (9)
|
NIUN |
1:02.56 |
1:04.96 |
2.40 |
| 21 |
Cabral, Aurora (10)
|
NIRRST |
1:04.08 |
1:06.69 |
2.61 |
| 22 |
Wagner, Keaghan J (9)
|
NISDSC |
1:03.71 |
1:08.45 |
4.74 |
| 23 |
Farley, Megan K (9)
|
NIGRSC |
|
1:09.58 |
- |
| 24 |
Strausser, Cadence A (9)
|
NIGRAT |
|
1:11.69 |
- |
| 25 |
Chen, Chloe Y (9)
|
NISDSC |
|
1:14.20 |
- |
|
Koneski Collette, Eliana J (10)
|
NIGRSC |
|
DQ |
- |
|
Crisco, Jacqueline E (9)
|
NIHAWK |
|
DQ |
- |
|
Olmstead, Hannah E (9)
|
NIVICT |
|
DQ |
- |