| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gilbert, Natalie J (10)
|
NIUN-V |
34.12 |
33.36 |
-0.76 |
| 2 |
Baum, Scarlett A (10)
|
NIGRSC |
41.84 |
38.88 |
-2.96 |
| 3 |
Conner, Haley M (9)
|
NIGRAT |
37.72 |
40.02 |
2.30 |
| 4 |
Jensen, Lillian M (10)
|
NIGRSC |
43.84 |
40.71 |
-3.13 |
| 5 |
Martin, Lily L (9)
|
NISDSC |
40.34 |
41.12 |
0.78 |
| 6 |
Wing, Gianna I (10)
|
NIGRSC |
51.52 |
42.44 |
-9.08 |
| 7 |
Walsh, Teagan M (10)
|
NIHAWK |
|
43.70 |
- |
| 8 |
Galley, Morgan C (10)
|
NIRRST |
47.44 |
44.32 |
-3.12 |
| 9 |
Borrelli, Kimberlee R (10)
|
NIGRSC |
44.12 |
45.02 |
0.90 |
| 10 |
Thaney, Brigid G (10)
|
NIGRSC |
46.39 |
45.23 |
-1.16 |
| 11 |
Kuby, Ashlynn M (9)
|
NISDSC |
43.98 |
46.52 |
2.54 |
| 12 |
Ayers, Brianna S (9)
|
NIGRSC |
49.33 |
47.53 |
-1.80 |
| 13 |
Magnera, Alaura (10)
|
NIWEBS |
48.34 |
48.48 |
0.14 |
| 14 |
Brett, Catherine M (9)
|
NIPACK |
47.52 |
48.96 |
1.44 |
| 15 |
Agostini, Ava E (10)
|
NIGRSC |
50.08 |
49.20 |
-0.88 |
| 16 |
Williams, Lauren K (9)
|
NIHAWK |
53.66 |
51.97 |
-1.69 |
| 17 |
Bermudez, Airianna L (10)
|
NIGRSC |
53.31 |
54.67 |
1.36 |
| 18 |
Cosner, Brynn S (10)
|
NIGRSC |
|
56.17 |
- |
| 19 |
McFadden Olivier, Camryn (9)
|
NIPACK |
1:00.01 |
1:02.20 |
2.19 |
|
Prasinos, Sofia G (10)
|
NIGRSC |
|
NS |
- |