| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Medlin, Mia G (7)
|
GUESA |
24.68 |
20.47 |
-4.21 |
| 2 |
Chien, Julienne Y (7)
|
GUCFSC |
23.56 |
21.13 |
-2.43 |
| 3 |
Steele, Makayla A (7)
|
GUSTA |
28.51 |
24.69 |
-3.82 |
| 4 |
Heredia, Amanda (7)
|
GUUN-C |
29.64 |
25.55 |
-4.09 |
| 5 |
Anda, Carrie (7)
|
GUCFSC |
29.06 |
26.92 |
-2.14 |
| 6 |
Figgs, Zoey A (7)
|
GUCFSC |
32.07 |
28.03 |
-4.04 |
| 7 |
Oquendo, Jorlaney M (7)
|
GUPACK |
40.07 |
28.97 |
-11.10 |
| 8 |
Weikel, Trinity G (7)
|
GUESA |
27.36 |
29.39 |
2.03 |
| 9 |
Gonzalez, Alyssa A (7)
|
GUPACK |
32.33 |
30.07 |
-2.26 |
| 10 |
Smith, Annalee R (7)
|
GUCFSC |
30.38 |
30.27 |
-0.11 |
| 11 |
Wheeler, Delaney R (7)
|
GUCFSC |
33.06 |
30.79 |
-2.27 |
| 12 |
Notaro, McKenzie A (7)
|
GUCFSC |
30.12 |
30.99 |
0.87 |
| 13 |
Betts, Ava G (7)
|
GUCFSC |
37.99 |
31.00 |
-6.99 |
| 14 |
Jones, Billie J (7)
|
GUNEHA |
33.92 |
31.92 |
-2.00 |
| 15 |
Bancroft, Sydney M (7)
|
GUPACK |
28.18 |
32.59 |
4.41 |
| 16 |
Adam, Genevieve J (7)
|
GUCFSC |
|
33.84 |
- |
| 17 |
Freed, Sophia A (7)
|
GUPACK |
28.75 |
34.87 |
6.12 |
| 18 |
Withers, Amelia E (7)
|
GUCFSC |
|
41.00 |
- |
|
D'Anna, Emma L (7)
|
GUCFSC |
|
DQ |
- |
|
Ramirez-Davila, Emma L (7)
|
GUPACK |
|
DQ |
- |
|
Coreas, Claire E (7)
|
GUPACK |
58.04 |
NS |
- |
|
Vaughan, Layla L (7)
|
GUPACK |
|
NS |
- |