| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lowe, Dalton D (16)
|
GUSST |
20.66 |
22.34 |
1.68 |
| 2 |
Baskett, Walter R (18)
|
GUSSAN |
21.46 |
22.78 |
1.32 |
| 3 |
Siemer, Spencer F (17)
|
GULSST |
22.51 |
22.89 |
0.38 |
| 4 |
McMahon, John D (16)
|
GUTYES |
24.17 |
23.41 |
-0.76 |
| 5 |
Van Lier, Jasper (16)
|
GUSSAN |
23.22 |
23.55 |
0.33 |
| 6 |
Mora, Alejandro M (16)
|
GUTYES |
22.85 |
23.81 |
0.96 |
| 7 |
Ward, Owen M (15)
|
GUSSAN |
22.87 |
23.82 |
0.95 |
| 8 |
Robertson, Jonathon L (16)
|
GUSSAN |
24.64 |
24.83 |
0.19 |
| 9 |
Kelly, Michael R (16)
|
GUSSAN |
24.23 |
24.86 |
0.63 |
| 10 |
Ward, Eli T (16)
|
GUSSAN |
24.63 |
25.01 |
0.38 |
| 11 |
Saria, Noah D (15)
|
GUTYES |
24.11 |
25.20 |
1.09 |
| 12 |
Martino, Henrique C (17)
|
GUSSAN |
23.29 |
25.23 |
1.94 |
| 13 |
Moon-Puente, Kalvin G (18)
|
GUTYES |
25.33 |
25.48 |
0.15 |
| 14 |
McWhorter, Alexander G (15)
|
GUTYES |
26.01 |
26.97 |
0.96 |
|
Isaacs, Tanner C (16)
|
GUSSAN |
|
NS |
- |
|
Irwin, Hunter E (16)
|
GUSSAN |
27.28 |
NS |
- |
|
Williams, Jayden B (15)
|
GUSST |
25.69 |
NS |
- |