| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tenhundfeld, Emma L (10)
|
OHKEY |
2:22.40 |
2:23.53 |
1.13 |
| 2 |
Lucas, Reese E (10)
|
OHCFY |
2:34.74 |
2:36.42 |
1.68 |
| 3 |
Schweikert, Maya L (9)
|
OHCY |
3:02.47 |
2:37.23 |
-25.24 |
| 4 |
Wagner, Zaida M (10)
|
OHCY |
2:36.28 |
2:38.62 |
2.34 |
| 5 |
Burke, Kathleen (10)
|
OHGMVY |
|
2:53.36 |
- |
| 6 |
Farnham, Alexandria E (10)
|
OHCY |
3:06.12 |
3:09.28 |
3.16 |
| 7 |
Caplinger, Katie (9)
|
OHHHY |
3:36.85 |
3:09.74 |
-27.11 |
| 8 |
Winkler, Anna E (10)
|
OHCY |
3:28.64 |
3:15.80 |
-12.84 |
| 9 |
Butler, Scarlett E (10)
|
OHCY |
|
3:15.87 |
- |
| 10 |
Roberts, Kara L (9)
|
OHCFY |
|
3:16.08 |
- |
| 11 |
Smith, Madison E (10)
|
OHKEY |
3:24.86 |
3:24.00 |
-0.86 |
| 12 |
Broaddrick, Lydia M (9)
|
OHCY |
|
3:27.79 |
- |
| 13 |
Shamleffer, Esme V (10)
|
OHCY |
|
4:12.34 |
- |
|
Cukovecki, Allisson M (10)
|
OHCY |
3:58.25 |
NS |
- |
|
Hayes, Catherine L (10)
|
OHKEY |
|
NS |
- |
|
Stone, Piper V (10)
|
OHKEY |
3:01.55 |
NS |
- |